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:Hot Start Taq DNA Polymerase

Hot Start Taq DNA Polymerase/HotStart Taq DNA Polymerase


 



The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3′end.The PCR product can be cloned in TA vector.
Applications:
Hot-start PCR amplification
Specific amplification of complex cDNA and genomic template
Amplification from low copy number DNA template
Real-Time PCR
Multiple PCR
Generation of PCR products for TA cloning

Hot Star Taq DNA Polymerase
Quality Control:
Functional absence of double and single-stranded endonuclease activity; Purity>99% test by SDS gel electrophoresis; Each lot of Hot Star Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA ; Retain full activity at room temperature for one week; No host DNA residue.
Concentration:  5 u/μl
Storage: Store at -20°C
Reaction Mixture Set Up
Package: This Hot Star Taq always supplied by Bulk package, if you ant the small package it is available.

 

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