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:Taq DNA Polymerase

This Taq DNA polymerase we supplied by Bulk package
Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus.The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity.The extending speed is 1-2 kb/min.There is an "A" on 3′end.The PCR product can be cloned in TA vector.
Conc. 5 U/μl  
Store at -20°C
Characteristics:
1.high-sensitivity 2.high amplification efficiency
Unit Definition:
One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-preceptible material in 30 minutes at 74°C.
Quality Control:
This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis.Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA .
Storage Buffer: 20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.
10X PCR reaction Buffer: 500 mM KCl,100 mM Tris-Cl (pH8.5 at 25℃),1% Triton X-100,15 mM MgCl2
Reaction Mixture Set Up
Component Volume                  Final Concentration
Template DNA <0.5 ug            as required
Forward Primer (10 μM) 1 μl   0.2-0.4 μM each
Reverse Primer (10 μM) 1 μl  0.2-0.4 μM each
10×PCR reaction Buffer 5 μl 1×
2.5 mM dNTPs  4 μl  0.2 mM
Taq DNA polymerase 0.5μl  2.5 unit
ddH2O to final volume 50μl  Not applicable
Recommended thermal cycling conditions
94℃ 2-5 min
94℃ 30 sec
50-60℃ 30 sec        30-35 cycles
72℃ 1-2 kb/min
72℃ 5-10 min

 

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