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:2*HotStar Taq Mastermix dye+

Description

The HotStar Taq Mastermix which include the HotStar DNA Polymerase,PCR Buffer, Mg2+ , dNTPs, the blue dye and the stabilizing agent. The HotStarTaq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3′end.The PCR product can be cloned in TA vector.

Applications:

Hot-start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiple PCR Generation of PCR products for TA cloning
Quality Control:
Functional absence of double and single-stranded endonuclease activity; Assayed for amplification from as little as 10 ng of human genomic DNA ; Retain full activity at room temperature for one week; No host DNA residue.
Storage: Store at -20°C

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