Ribonuclease R, RNase R
2022-09-15Klenow Fragment (3′-5′ exo-)
2022-09-15Storage Condition
-20°C
Components
- HotVent exo- DNA Polymerase 2U/μl
- 10x HotVent exo- DNA Polymerase
- Reaction Buffer
Description
HotVent exo- DNA Polymerase purified E. coli strain of self weight group. This enzyme is a natural enzyme obtained by genetic engineering. The polymerase is a high fidelity heat-resistant DNA polymerase with a half-life of 8 hours at 100°C. It is suitable for primer extension and high temperature (72°C) DNA sequencing.
Unit Definition
At 75°C, within 30 minutes, the amount of enzyme required for the incorporation of 10 nmol of whole nucleotides into acid insoluble precipitates is defined as 1 active unit (U).
Quality Control
HotVent exo- DNA Polymerase SDS-PAGE purity > 99%, no endonuclease and exonuclease activities.
Precautions
- In general, the annealing temperature is 5°C lower than the fusion temperature Tm of the amplification primer in the experiment. When the ideal amplification efficiency cannot be obtained, the annealing temperature should be appropriately reduced; When nonspecific reaction occurs, the annealing temperature is increased to optimize the reaction conditions.
- The extension time should be set according to the size of the amplified fragment. The amplification efficiency of HotVent exo- DNA Polymerase is 1 KB / min.
- The number of cycles can be set according to the downstream application of amplification products. If the number of cycles is too small, the amplification amount is insufficient; If the number of cycles is too many, the mismatch probability will increase, and the non-specific background will be serious. Therefore, the number of cycles should be minimized on the premise of ensuring the yield of products.
Order
| HV01 | HotVent exo- DNA Polymerase | 2U/μl |
