Product Number: RNK0902
Shipping and Storage
1.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2.To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK0902 50 Preps |
| Buffer RLT | 4°C | 50 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| PLANTaid | RT | 5 ml |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
The unique Buffer RLT rapidly cleaves cells and inactivates cell RNA enzymes. The plant RNA co extractant PLANTaid helps to bind to polysaccharide polyphenols and remove them by centrifugation. Then, after adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins, Finally, low salt RNase free H2O elutes pure RNA from the silicon substrate membrane.
Features
1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2.Simplicity, single sample operation can generally be completed within 25 minutes, making it the simplest and fastest reagent kit in the world.
3.The unique plant RNA extractant PlantAid can effectively bind polysaccharides and polyphenols, improving clearance efficiency.
4.The world's advanced adaptability is extremely extensive, and it can extract hundreds of samples that have failed to be extracted by domestic and foreign reagent kits, including cotton, roses, Arabidopsis, poplar, and so on. For a detailed sample list, please refer to the product introduction on the company homepage.
5.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of up to 2.1-2.2 and minimal DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
