Virus Genomic DNA/RNA Fast Kit II（NP）
2024-11-29EASYspin Plus Bacterial Fast RNA Kit
2024-11-29EASYspin RNA Plant Mini Kit
Product Number: RNK0902
Shipping and Storage
1.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2.To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK0902 50 Preps |
| Buffer RLT | 4°C | 50 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| PLANTaid | RT | 5 ml |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
The unique Buffer RLT rapidly cleaves cells and inactivates cell RNA enzymes. The plant RNA co extractant PLANTaid helps to bind to polysaccharide polyphenols and remove them by centrifugation. Then, after adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins, Finally, low salt RNase free H2O elutes pure RNA from the silicon substrate membrane.
Features
1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2.Simplicity, single sample operation can generally be completed within 25 minutes, making it the simplest and fastest reagent kit in the world.
3.The unique plant RNA extractant PlantAid can effectively bind polysaccharides and polyphenols, improving clearance efficiency.
4.The world's advanced adaptability is extremely extensive, and it can extract hundreds of samples that have failed to be extracted by domestic and foreign reagent kits, including cotton, roses, Arabidopsis, poplar, and so on. For a detailed sample list, please refer to the product introduction on the company homepage.
5.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of up to 2.1-2.2 and minimal DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
Note
1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000 rpm.
2.Self prepared ethanol and mortar (optional) are required.
3.Buffer RLT and Buffer RW1 contain irritating compounds. Wear latex gloves during operation to avoid contact with skin, eyes, and clothing. If it gets on the skin or eyes, rinse with plenty of water or physiological saline.
4.Regarding trace residues of DNA:
Generally speaking, any total RNA extraction reagent cannot completely avoid trace DNA residues during the extraction process. Our company's EASYspin series RNA extraction products, due to the use of our unique buffer system and the selection of adsorption membranes with special adsorption capabilities, have removed the vast majority of DNA residues. In most RT-PCR amplification processes, the impact of extremely small amounts of DNA residues is not significant, If strict mRNA expression analysis, such as fluorescence quantitative PCR, is required, we recommend that when selecting templates and primers:
4.1.Select primers that cross introns to pass through the connecting regions in mRNA, so that DNA cannot serve as a template for amplification reactions.
4.2.Select primer pairs with different sizes of amplified products on genomic DNA and cDNA.
4.3.Treat the RNA extract with DNase I of RNase free. This reagent kit can also be used for RNA cleaning after DNase I treatment. Please contact us for specific operating instructions.
4.4.Before rinsing buffer RW1, perform DNase I column digestion directly on the adsorption column RA. Before purchasing the DNase Digestion Kit (Product Number: RNK3401), you can first request specific operating instructions.
5.There are two options for extracting residual DNA from complex plant samples:
5.1.Before rinsing buffer RW1, perform DNase I column digestion directly on the adsorption column RA. Before purchasing the DNase Digestion Kit (Product Number: RNK3401), you can first request specific operating instructions.
5.2.When extracting some complex plant samples, there may be a lot of DNA residue. You can try our company's RNK3802: EASYspin Plus Plant Fast RNA. On the basis of RNK0902, RNK3802 has independently developed the genome DNA clearance column technology, which can effectively remove DNA residues. In most cases, DNA residues can be cleared until they are invisible under UV observation.
6.Regarding the situation where the extraction of plant samples with particularly complex and difficult extraction fails or the yield is low:
Some particularly complex plant samples, such as rice seeds, grape fruits, indigo fruit fruits, lily bulbs, potato tubers, etc., cannot be extracted using RNK0902: Buffer RLT, so RNK5301: EASYspin Plus complex Plant RNA Kit needs to be selected. Some samples have lower yields, but RNK5301: EASYspin Plus complex Plant RNA Kit can also be attempted. RNK5301 is equipped with a powerful Buffer RLTCLB option, which can extract complex samples or significantly increase production in many cases (please refer to the RNK5301 manual for details).
