Virus Genomic DNA/RNA Fast Kit II (PK)

Product Number: RNK3201

Shipping and Storage 

To avoid reducing activity and facilitate transportation, Proteinase K is provided as a freeze-dried powder. Upon receipt, it can be briefly centrifuged and dissolved in 1ml of sterilized water. Because repeated freeze-thaw cycles may reduce enzyme activity, immediately after dissolution, pack and freeze according to the amount used each time (20µl), and store at -20℃.

Components

Component	Storage	RNK3201 50 Preps	RNK3202 100 Preps
Buffer VCB	RT	10 ml	20 ml
Poly Carrier	-20℃	100μl	200μl
Buffer RE	RT	25 ml	50 ml
Buffer RW	RT	10 ml	10 ml×2
Proteinase K(20mg/ml)	-20℃	20 mg	20 mg×2
RNase-free H2O	RT	10 ml	10 ml
Adsorption column RA and collection tube	RT	50 sets	100 sets

Storage at room temperature for 12 months does not affect the effectiveness of use, and each solution should be covered tightly in a timely manner after use.

Description

The Virus Genomic DNA/RNA Fast Kit II(PK) is suitable for rapid extraction of high-purity viral DNA/RNA from acellular body fluids, including plasma, serum, ascites, cultured cell supernatant, cerebrospinal fluid, and urine, using a centrifugation adsorption column that specifically binds to viral DNA/RNA and a unique buffer system. This product can meet the simultaneous extraction requirements of most viral RNAs/DNA, such as viral RNAs: HCV (hepatitis C virus), HIV (HIV), and HTLV (human T-lymphotropic virus); Virus DNA: HBV (hepatitis B virus) and CMV (cytomegalovirus), etc. After virus lysis, DNA/RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state (especially equipped with Poly Carrier, which can easily capture trace amounts of nucleic acids from the system). Then, impurities such as salt, cellular metabolites, and proteins are removed through a series of rapid rinsing centrifugation steps. Finally, the pure virus DNA/RNA is eluted from the silica matrix membrane using a low salt elution buffer. The purified viral nucleic acid is free of impurities and PCR inhibitors, and can be directly used for PCR/RT-PCR analysis.

Feature

1.No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.

2.Time saving, concise, and single sample operation can generally be completed within 30 minutes.

3.Multiple column washes ensure high purity, and the extracted virus DNA/RNA has high purity, stable and reliable quality. It can be used for various routine operations, including PCR/RT-PCR, enzyme digestion, sequencing, Southern hybridization, etc.

Note

1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000rpm.

2.Preheat the required water bath to a specific temperature before starting the experiment.

3.Poly Carrier:

4.Usage of Poly Carrier

If the initial processing volume is small, we recommend using Poly Carrier. If a large amount of nucleic acid production is expected, users can choose whether to join Poly Carrier according to their needs. When using, add 2µl Poly Carrier storage solution to the Buffer VCB required for each sample extraction, and mix the Buffer VCB and Poly Carrier solution completely upside down (Buffer VCB is easy to form foam, do not use vortex oscillation to mix). You can also add the required Poly Carrier to the total required Buffer VCB according to the number of samples and mix well for later use. The mixture is stable at room temperature for 24 hours.

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