Product Number: DNK3102
Shipping and Storage
1.When the ambient temperature is low, some detergent ingredients in Buffer NLY will precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
2.Proteinase K is stored in a ready-to-use glycerol buffer and transported at room temperature. Upon receipt, it should be stored at room temperature for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years
3.Avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air. Each solution should be covered tightly after use.
Components
| Component | Storage | DNK3102320Preps×50µl |
| Buffer NLY | RT | 180 ml |
| Buffer PP | RT | 70 ml |
| Glycogen | -20℃ | 0.7 ml |
| Buffer DA | RT | 10 ml |
| Proteinase K | 4℃ | 1 ml |
Description
This reagent kit is exclusively developed based on the characteristics of whole blood coagulation. Buffer NLY is combined with Proteinase K to lyse and coagulate blood clots to release genomic DNA. Then, Buffer PP selectively precipitates to remove proteins. Finally, pure genomic DNA is precipitated in isopropanol with the assistance of molecular biology grade Glycogen and re dissolved in Buffer DA.
Features
1.No need to use toxic reagents such as phenol.
2.Fast and simple, the operation of a single sample can generally be completed within 1 hour.
3.The results are stable, and Glycogen helps precipitate trace amounts of DNA with high yield. The typical OD260/OD280 ratio is 1.7-1.9, and the length can reach 50kb-150kb. It can be directly used for library construction, PCR, Southern blot, and various enzyme digestion reactions.
