Blood DNA Max kit
2024-12-02Tissue/cell genomic DNA Extraction Kit
2024-12-02Product Number: DNK0701
Shipping and Storage
- Buffer TL and Buffer CB may precipitate and precipitate at low temperatures. They can be dissolved again in a 37℃ water bath for a few minutes to restore clarity. After cooling to room temperature, they can be used.
- Proteinase K is stored in a ready-to-use glycerol buffer and transported at room temperature. Upon receipt, it should be stored at room temperature for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years
- Avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air. Each solution should be covered tightly after use.
Components
| Component | Storage | DNK0701 50 Preps | DNK0702 100 Preps | DNK0703 200 Preps |
| Balance Buffer | RT | 5 ml | 10 ml | 20 ml |
| Buffer TL | RT | 11 ml | 20 ml | 40 ml |
| Buffer CB | RT | 11 ml | 20 ml | 40 ml |
| Buffer PE | RT | 16 ml | 32 ml | 64 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 20 ml |
| Proteinase K | 4°C | 1 ml | 1 ml×2 | 1 ml×4 |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The unique Buffer CB/Proteinase K rapidly lyse cells and use silica gel membrane centrifugation columns to specifically adsorb DNA, without the need for toxic reagents such as phenol chloroform or time-consuming alcohol precipitation, maximizing the removal of proteins and other inhibitory impurities. Suitable for efficiently extracting genomic DNA from various materials such as animal tissue cells, mouse tails, insects, etc. The extracted DNA can be directly used for experiments such as enzyme digestion, PCR, Southern Blot, and virus detection.
Features
- There is no need to use toxic reagents such as phenol, nor do steps such as ethanol precipitation.
- Fast and simple, single sample operation can generally be completed within 30 minutes.
- Multiple column rinsing ensures high purity, with a typical OD260/OD280 ratio of 1.7-1.9 and a length of up to 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
Application
Suitable for extracting genomic DNA from tissues/cells/mouse tails/insects.
