Circulating Nucleic Acid Mini Kit

Product Number: DNK4701

Shipping and Storage 

1.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).

2.Proteinase K is stored in a ready to use glycerol Buffer and transported at room temperature. After receipt, store at room temperature not exceeding 20℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.

3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	DNK4701 50 Preps
Buffer ACL	RT	40 ml
Buffer ACB	RT	60 ml
Buffer PE	RT	16 ml
Buffer RW	RT	10 ml
RNase-free H2O	RT	5 ml
Proteinase K	4°C	5 ml
RNase free adsorption column RA and collection tube	RT	50 sets

Description

This reagent kit is suitable for extracting free circulating nucleic acid DNA/RNA from fresh or frozen (frozen but not thawed, freeze-thaw will affect quality) serum, plasma, urine, and other cell-free body fluids. This reagent kit has developed a unique Buffer system for the small and low content of free circulating nucleic acid fragments, ensuring efficient extraction of free nucleic acid. The purified free nucleic acid has high yield and good quality, and can remove proteins, pigments, lipids, and other inhibitors to the maximum extent. This product can handle 0.1-1 ml of liquid samples, and the elution volume of the configured high-efficiency micro adsorption column can be as low as 20μl. The free nucleic acid content is extremely low (typical concentration of 1-100ng/ml plasma), and the yield is closely related to the type of sample, storage conditions, time, and individual differences. The purified free DNA has stable and reliable quality, and can be directly used in molecular biology experiments such as PCR, fluorescence quantitative PCR, and second-generation sequencing.

Features

1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.

2.Simplicity, single sample operation can generally be completed within 30-50 minutes.

3.The purified free nucleic acid has high yield and good quality, and performs better in downstream experiments.

Application

Suitable for extracting free circulating DNA/RNA and viral nucleic acid DNA/RNA from human plasma, serum, urine, or other cell-free body fluids.

Note

1.After the sample is frozen and stored until extraction, thawing should be avoided. If it is indeed unavoidable, multiple freeze-thaw cycles should be avoided, otherwise it may lead to a decrease in extraction volume.

2.It is recommended to centrifuge at 16000g for 5-10 minutes at 4℃ before treatment to further remove cell debris and reduce contamination of gDNA and RNA from damaged blood cells. If there is a small protein precipitation after freezing, it is also recommended to centrifuge at 4℃ at 16000g for 5 minutes.

3.Self prepared ethanol and isopropanol are required. Please preheat the water bath to 60℃ before star ting the experiment.

4.This reagent kit can extract 0.1-1 ml of liquid samples.

5.If the sample is processed as urine, please contact us and order Buffer ATL separately.

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