Manual

Product Number: MB04T

Product Description

MB04T are monodisperse ferrimagnetic beads, ~1µm in diameter. The magnetic beads ensure rapid magnetic mobility and efficient isolation of high-purity mRNA from eukaryotic cells total RNA, cell and crude extracts of animal and plant tissues.

The mRNA can be directly used in most downstream applications of molecular biology, like RT-PCR, solid-phase cDNA library

construction, S1 enzyme analysis, RNase protection assay, primer extension, dot-blot hybridization, in vitro translation, RACE, subtractive hybridization, Northern analysis, gene cloning and expression analysis.

Oligo (dT)30 magnetic microspheres were designed based on the principle of base complementary pairing and poly (A) tail in mRNA. After annealing, place the reaction tube on a magnetic frame, then the mRNA binding to the magnetic microspheres was concentrated and collected, then discard the supernatant containing impurities. The protocol can be carried out in 15 minutes without preparation for total RNA. Oligo (dT)30 on the surface of magnetic microspheres can be used to capture mRNA, and the first strand cDNA can be synthesized with oligo (dT)30 as primer under the action of reverse transcriptase.

Description of Materials

1. Diameter:~0.7µm
2. Concentration:10mg/ml
3. Surface group:Oligo(dT)₃₀
4. Density: 1.4g/cm³
5. Storage Buffer: PBS pH 7.4, 0.1% Proclin 300

Binding ability

10μg mRNA can be isolated per 100 μL (1 mg) magnetic microspheres, depending on the type of tissue or cell and the level of

mRNA expression.

Storage and Stability

Store at 2℃ to 25℃ for three years.

Recommended application

mRNA extraction

Limited Product Warranty

Freezing, drying, centrifuging, etc. of the product may cause irreversible agglomeration of the microspheres, resulting in decreased bio-application performance.

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