DNase I ,RNase free kit
2024-10-24
Magnetic Bead DNA Purification Kit (for NGS Size Selection)
2024-10-24Product Number: DNK2361
Shipping and Storage
Temperature (15-30°C)
Components
| Component | DNK2361 96 preps | DNK2361 4×96 preps |
| Buffer ML | 24 mL | 96 mL |
| Buffer GW1(concentrate) | 80 mL | 4×80 mL |
| Buffer GW2(concentrate) | 50 mL | 4×50 mL |
| Buffer EB | 30 mL | 96 mL |
| Proteinase K | 2×25 mg | 180 mg |
| Proteinase K Storage Buffer | 2×1.25 mL | 2×5 mL |
| Magbeads PN | 2×1 mL | 8 mL |
Description
The kit provides a simple, rapid, and efficient method for blood DNA extraction from fresh or frozen anticoagulant blood (citrate, EDTA, heparin treated blood samples). In the presence of high salt, DNA binds to the surface of the silicon-based coated Magbeads. After rinsing, the highly purified DNA is eluted in BufferEB or deionized water. DNA yield depends on the type of sample, storage conditions, time, and the content of white blood cells in the sample. 7-10 μg genomic DNA can usually be extracted from 200 μL of cryopreserved anticoagulant blood. The purified DNA has good purity (A260/280 ratio between 1.8-2.0, A260/230 ratio more than 1.5-2.5) and high integrity (up to 150 kb), which can be used for next-generation sequencing, cloning, quantitative PCR, chip detection and other downstream experiments.
The kit can be used with liquid workstation and magnetic rod method automatic magnetic bead extraction system, simple and rapid large-scale extraction, greatly reducing the experimenter's workload and human error in the experiment.
