Selectively Apoptotic DNA Ladder Extract Kit
2024-12-05Yeast Genomic Fast Kit
2024-12-05Product Number: DNK1801
Components
| Component | Storage | DNK1801 100 Preps |
| Buffer SL | 4℃ | 50 ml |
| 100×Buffer DC | 4 ℃,avoid light | 0.5 ml |
| Buffer DD | 4°C or RT | 50 ml |
Description
The morphological changes of cells in the middle and late stages of apoptosis are chromatin aggregation and consolidation in local areas, followed by nuclear fragmentation and the appearance of apoptotic bodies. Under Hoeschst staining, the DNA of the nucleus or apoptotic bodies will exhibit dense staining or fragmented dense staining. The excitation wavelength of Hoechst dye ultraviolet light in this reagent kit is 350-370nm; The emission wavelength is 465nm, and DNA emits blue fluorescence under a fluorescence microscope.
Note
- A fluorescence microscope or laser confocal microscope capable of observing blue fluorescence is required.
- PBS or 0.9% NaCl solution, cover glass and slide are required.
- Fluorescence is easily quenched, so it should be handled and stored away from light as much as possible.
