Yeast Genomic Fast Kit
2024-12-05λ Phage Genome Kit
2024-12-05Product Number: DNK2101
Shipping and Storage
- Buffer MB may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | DNK2101 50 Preps | DNK2102 100 Preps | DNK2103 200 preps |
| Buffer MB | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 15 ml | 25 ml | 2×25 ml |
| Buffer EB | RT | 10 ml | 20 ml | 40 ml |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
M13 and other filamentous phage vectors are very useful in library construction and providing single stranded DNA and inducing mutations for sequence sequencing. Centrifuge an appropriate amount of liquid culture infected with M13 filamentous bacteriophages or related phages (M13 source). The single-stranded bacteriophage DNA in the supernatant selectively adsorbs onto the silica matrix membrane in a high dissociation salt state. Then, through a series of rapid rinsing centrifugation steps, impurities such as salt, cell metabolites, and proteins are removed. Finally, the pure bacteriophage single-stranded DNA is eluted from the silica matrix membrane by low salt Buffer EB.
Features
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Time saving, concise, and single sample operation can generally be completed within 10 minutes.
- High yield, typical production of 800μl M13 filamentous bacteriophage supernatant can extract 3μg of phage single stranded DNA.
- Multiple column washes ensure high purity, with a typical OD260/0D280 ratio of 1.7 to 1.9. It can be directly used for sequencing, typically with a recognizable reading of up to 650bp.
Application
Suitable for rapid extraction of M13 phage single stranded DNA
