Circulating Nucleic Acid Mini Kit
2024-12-10PCR Purification Kit
2024-12-10Product Number: DRK0101
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity. It should be restored to room temperature before use.
- Storing at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DRK0101 50 Preps | DRK0102 100 Preps | DRK0103 200 Preps |
| Balance Buffer | RT | 5 ml | 10 ml | 20 ml |
| Buffer DD | RT | 40 ml | 75 ml | 150 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 10 ml | 15 ml | 15 ml |
| Adsorption column EC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
In the presence of high dissociation salts, DNA fragments selectively adsorb onto the silica matrix membrane inside the centrifuge column. Through a series of rapid rinsing centrifugation steps, Buffer WB removes impurities such as primers, nucleotides, proteins, enzymes, etc. Finally, low salt, high pH Buffer EB elutes pure DNA from the silica matrix membrane.
Features
- The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- High quality buffer DD was used, which does not contain traditional buffer DD's sodium iodide and perchlorate, and does not inhibit downstream reactions such as enzyme digestion and cloning after recovery.
- Buffer DD is modulated with phenol red to become a yellow color, which facilitates observation of sol effect and monitoring of pH changes to achieve optimal binding effect, greatly improving recovery efficiency.
- The improved Buffer DD formula greatly enhances buffering capacity and stability, allowing pH to be buffered within the optimal binding range even with significant sample changes.
- Fast and convenient, without the need for toxic reagents such as phenol and chloroform, and without the need for ethanol precipitation.
Application
Suitable for DNA recovery from agarose gel
