Manual

Product Number: RNK0801

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2. Improper storage at low temperatures (4℃ or -20℃) can cause solution precipitation and affect the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

The unique Buffer RLT rapidly cleaves cells and inactivates cellular RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing and centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cellular metabolites and proteins. Finally, low salt RNase free H₂O washes pure RNA off the silica matrix membrane

Features

1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. No toxic reagents such as phenol and chloroform are needed, and no steps such as ethanol precipitation are required.
3. Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
4. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.0 and minimal DNA residue, which can be used for RT-PCR, Northern blot, and various experiments.

Application

Suitable for rapid extraction of total bacterial RNA

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