Product Number: RNK3701
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
- Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | RNK3701 10preps |
| Buffer RLT | RT | 100 ml |
| Buffer RW1 | RT | 120 ml |
| Buffer RW | RT | 25ml×2 |
| RNase-free H2O | RT | 10 ml |
| PLANTaid | 4℃ | 10 ml×2 |
| RNase free adsorption column RA and collection tube | RT | 10 |
Description
Unique Buffer RLT/ β-Mercaptoethanol rapidly cleaves cells and inactivates cell RNA enzymes. Plant RNA extractant PLANTaid helps to bind to polysaccharide polyphenols and remove them by centrifugation. Then, after adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. A series of rapid rinsing centrifugation steps, such as Buffer RW1 and Buffer RW, are used to remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O elutes pure RNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the unstable membrane quality of domestic reagent kits.
- No toxic reagents such as phenol and chloroform are needed, and no steps such as ethanol precipitation are required.
- Fast and simple, the operation of a single sample can generally be completed within 60 minutes.
- The unique plant RNA extractant can effectively bind polysaccharides and polyphenols, improving the clearance effect.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.0 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
Applications
Suitable for rapid and large-scale extraction of total RNA from plant tissue cells
