Product Number: RNK3802
Shipping and Storage
- Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK3802 50Preps |
| Buffer RLT | RT | 50 ml |
| Buffer CLB（presenter） | RT | 8 ml |
| Buffer RLT Plus | RT | 25 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| PLANTaid | RT | 5 ml |
| Genomic DNA clearance column and collection tube | RT | 50 |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
On the basis of our company's exclusive introduction of EASYspin phenol free and chloroform based rapid RNA extraction technology, we have also independently developed the genome DNA clearance column technology, which can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescence quantitative PCR and other experiments. The unique Buffer RLT rapidly cleaves cells and inactivates cellular RNA enzymes. The plant RNA co extractant PLANTaid helps to bind to polysaccharide polyphenols and remove them by centrifugation. Then, the mixture is lysed and the RNA binding is regulated by ethanol to adsorb onto the genomic DNA scavenging column. The RNA is selectively washed and filtered, and the residual DNA adsorbed on the genomic DNA scavenging column cannot be washed away. The column is discarded together to remove the DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O washes the pure RNA off the silica matrix membrane.
Features
- No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
- Simplicity, single sample operation can generally be completed within 25 minutes, making it the simplest and fastest reagent kit in the world.
- The unique plant RNA extractant PLANTaid can effectively bind polysaccharides and polyphenols, improving clearance efficiency.
- The independently developed genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.
- The world's leading adaptability is extremely extensive, and it can extract hundreds of samples that have failed to be extracted by domestic and foreign reagent kits, including cotton, roses, Arabidopsis, poplar, and so on.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.1~2.2 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
Application
Suitable for rapid extraction of total RNA from plant tissue cells, unique genomic DNA clearance column technology can effectively remove visible gDNA residues on electrophoresis. RNA can be used for reverse transcription PCR, fluorescence quantitative PCR, etc.
