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2025-02-14Detection Kit for Human Papilloma Virus 16/18 DNA
2025-02-17Diagnostic Kit for Human Papilloma Virus 14 High-Risk Genotyping DNA (Real-Time PCR Method)
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Product Number: QDHPV14
Shipping and Storage
- Store below 30°C. It is valid for 12 months.
- Transport at normal temperature, not suggested over 14 days. Opened but not completely used HPV PCR Master Mix should be stored at (-20±5) °C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.
- Date of manufacture and term of validity: see the label.
Components
| Reagent | QDHPV14 48T |
| HPV PCR Master Mix 1 | Lyophilized powder |
| HPV PCR Master Mix 2 | Lyophilized powder |
| HPV PCR Master Mix 3 | Lyophilized powder |
| HPV PCR Master Mix 4 | Lyophilized powder |
| Positive Control | Lyophilized powder |
| Negative Control | 1.0mL |
| Redissolved Diluent | 1.5mL×4 |
Note：1.Do not mix reagents from different batches.
2.The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, primers, probes, dNTPs, and Mg2+.
3.Add 100μL Redissolved Diluent to the Positive Control, mix well, and use a handheld centrifuge to centrifuge briefly before use.
Description
This kit uses real-time fluorescence PCR detection technology to achieve qualitative detection of HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 in genitourinary tract secretion samples by using a pair of specific primers and a specific fluorescent probe in the conserved region shared by HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68.The kit is provided with an Internal Control(IC), which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.
The kit contains 4 bottles. The specific test items are as follows:
| Contents | Test item | Detection Channel |
| HPV PCR Master Mix 1 | HPV 16 | FAM |
| HPV 31 | VIC(HEX) | |
| HPV 56 | ROX(TEXAS RED) | |
| HPV 52 | CY5 | |
| HPV PCR Master Mix 2 | HPV 18 | FAM |
| HPV 45 | VIC(HEX) | |
| HPV 59 | ROX(TEXAS RED) | |
| HPV 58 | CY5 | |
| HPV PCR Master Mix 3 | HPV 33 | FAM |
| HPV 35 | VIC(HEX) | |
| HPV 68 | ROX(TEXAS RED) | |
| HPV 66 | CY5 | |
| HPV PCR Master Mix 4 | HPV 39 | FAM |
| HPV 51 | VIC(HEX) | |
| Internal Control(IC) | ROX(TEXAS RED) |
Application
This kit is suitable for qualitative detection of 14 high-risk HPV DNA (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) in male urethral swab samples and female cervical swab samples in vitro.
Cervical cancer (CC) is the fourth most frequently diagnosed cancer and the fourth leading cause of cancer death in women worldwide. It has been demonstrated that persistent infection with various human papillomavirus (HPV)genotypes plays a major role in the development of high and low-grade cervical intraepithelial neoplasia (CIN) and CC. Human papilloma virus (HPV) is a small, double-stranded, circular DNA virus. HPV infection is considered the most common viral sexually transmitted infection worldwide. HPV has been classified genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 as carcinogenic to humans. The test results can be used for auxiliary diagnosis of sexual transmission and reproductive tract infectious diseases caused by human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68. The test results are only for clinical reference and should not be used as the sole criterion for clinical diagnosis.
