Foot-and-mouth Disease Virus Detection Kit (Real-Time PCR Method)
2025-02-19Mycobacterium bovis (MB) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-03-04Product Number: RNK4501LY
Shipping and Storage
Freeze dried powder is stored at 4 ℃, and after reconstitution, the solution is stored at -20 ℃
Components
| Component | RNK4501LY 2000U |
| DNase I, lyophilized powder | 6 |
| Reaction Buffer (with MgCl2), 10× | 6×1mL |
| 200 mM EDTA | 6×1mL |
| RNase Free Water | 12×1mL |
Description
DNase I is a deoxyribonuclease that requires a divalent cation and can be used to degrade single stranded or double stranded DNA. The principle is that DNase I hydrolyzes phosphate diester bonds to produce single nucleotides or oligonucleotides with 5 '- phosphate groups and 3' - OH groups. Both Mg2+ and Mn2+ can activate the activity of DNase I, while Ca2+ concentration directly affects enzyme activity. When Mg2+ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn2+, double stranded DNA can be broken and fragmented. Used for the preparation of RNA without DNA contamination, reverse transcription, and in vitro transcription experiments.
