Fowlpox Virus (FWPV) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-03-13
96-Well Extraction Plate
2025-03-13Product Number: DTK550
Shipping and Storage
Low temperature transportation, stored at -20℃, with a shelf life of 12 months. Positive controls need to be placed separately due to their tendency to contaminate other components. This product does not provide live samples as positive controls, only nucleic acid fragments as positive controls.
Component
| Component | Specification | Nine hole box packaging |
| 2×Probe qPCR MagicMix | 500μL | Brown cover |
| 10 × qRT-PCR enzyme mixture | 100μL | Red cover |
| Fluorescent PCR specific template diluent | 1mL | Green cover |
| Vesicular stomatitis virus VSV-G dye based qRT-PCR primer mixture | 100μL | White cover |
| Vesicular stomatitis virus VSV-G dye qRT-PCR positive control (1 × 10E7 copy/μL) | 50μL | Yellow cover |
Description
Vesicular stomatitis is a zoonotic infectious disease caused by a virus. It is mainly manifested in the occurrence of blisters on the oral mucosa, occasionally on the hoof crown and interphalangeal skin, and the appearance of foam like things. Infected animals are the main source of transmission for this disease. The initial manifestation of diseased animals is an increase in body temperature, followed by the appearance of blisters in the mouth and hooves. After the blisters rupture, the wound is not easily healed.
Application
This product is developed based on the principle of dye based fluorescence quantitative PCR, specifically for the detection of vesicular stomatitis virus VSV-G. It has the following characteristics:
- One stop shop, users do not need to prepare each ingredient separately, including primers and controls.
- Primers designed based on the conserved gene sequence of the vesicular stomatitis virus VSV-G genome have good specificity.
- Based on dye based qRT-PCR detection, the sensitivity is 10-100 times higher than conventional RT-PCR.
- Using one tube qRT-PCR technology, RT and PCR are completed in one test tube, without the need for intermediate sample transfer, reducing operational errors and potential contamination.
- This product is sufficient for 50 rounds of RT-PCR in a 20μL system and can only be used for scientific research, not for clinical use.
Specimen collection
Sample DNA.
