Chicken Infectious Anemia Virus (CIAV) Nucleic Acid Detection Kit (Fluorescent PCR Method)
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Product Number: DTK004
Shipping and Storage
- -20℃±5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
- The collected or processed samples should be stored at 2℃~8℃ for no more than 24 hours; If long-term storage is required, it should be stored at -70℃ or below, with no more than 3 freeze-thaw cycles.
Component
| Component | 50T |
| CIAV reaction solution | 500μL×2 |
| Enzyme solution | 50μL |
| CIAV positive quality control product （4.12×105copies/mL） | 250μL |
| Negative quality control product | 250μL |
Note: Different batches of reagents cannot be mixed.
Description
This kit uses TaqMan probe method for real-time fluorescence PCR technology, designs a pair of chicken infectious anemia virus specific primers, and combines them with a specific probe to amplify and detect the nucleic acid of chicken infectious anemia virus in vitro using fluorescence PCR technology, which is used for pathogenic diagnosis of suspected infectious materials in clinical practice.
Application
This kit is suitable for detecting chicken infectious anemia virus in samples such as thymus and bone marrow, and is used for auxiliary diagnosis of chicken infectious anemia virus infection. The test results are for reference only.
Specimen collection
CIAV exists in tissues and organs such as the liver, skin, spleen, thymus, bursa of Fabricius, kidneys, and bone marrow of diseased chickens. Aseptically collect the above tissues, prepare a 20% tissue suspension using serum-free DMEM, centrifuge at 3000 r/min for 30 minutes, and collect the supernatant. Other sample collection can refer to SNT4053-2014.
