Description

Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.

RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.

A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
• Concentration: 10 mg/mL
• Activity: ≥ 350 U/μL
• Molecular weight: 14.3 kDa
• Form: Liquid: clear colorless buffered aqueous glycerol solution
• Grade: Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected
• NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)
• Shelf life: 1 year (-20°C)

Transportation and Storage

Storage temperature: -20°C.
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).

Procedure

RNase A is provided as a solution of 10 mg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.

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