Product Number: DNK2201
Shipping and Storage
- Add 1ml of Buffer LS to RNaseA and DNaseI tubes respectively, blow and mix well, fully dissolve RNase A and DNase I, and then pack them into -20℃ freezer according to the amount used each time. The shelf life is 6 months.
- When the Buffer LB is at low temperature, precipitation and precipitation may occur. It can be dissolved again in a water bath at 37℃ for a few minutes to help restore clarity and transparency. After cooling to room temperature, it can be used.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | DNK2201 50 Preps | DNK2202 100 Preps |
| RNase A | -20℃ | 20 mg | 20 mg×2 |
| DNase I | -20℃ | 50 mg | 50 mg×2 |
| Buffer BP | RT | 100 ml | 100ml×2 |
| Buffer LS | RT | 30 ml | 60 ml |
| Buffer LP | RT | 5 ml | 10 ml |
| Buffer LB | RT | 20 ml | 40 ml |
| Buffer WB | RT | 15 ml | 25 ml |
| Buffer EB | RT | 10 ml | 15 ml |
| Adsorption column AC | RT | 50 | 100 |
| Collection tube (2ml) | RT | 50 | 100 |
Description
λ Phage vectors are widely used for library screening, and the purpose is to clone and cultivate a large number of bacterial particles that need to be extracted λ Perform subsequent work such as sequencing using bacteriophage DNA. λ The supernatant of phage lysate culture after centrifugation is first digested with a mixture of RNase A/DNase I enzymes to remove residual host bacterial DNA/RNA, and then precipitated to collect phages. The phages are lysed by SDS, and the remaining fragments are removed by precipitation centrifugation. The incoming bacteriophage DNA in the supernatant of the lysate is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state, followed by a series of rapid rinsing centrifugation steps to remove impurities such as salt, cellular metabolites, and proteins. Finally, the low salt Buffer EB washes the incoming bacteriophage DNA off the silica matrix membrane.
Features
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Time saving and simple, it can be used for the extraction of liquid culture lysates and solid culture plates. The operation of a single sample can generally be completed within 1.5 hours.
- High yield, typical yield of 10ml λphage lysate culture supernatant can extract about 10μg λphage DNA.
- Multiple column washes ensure high purity, with a typical OD260/0D280 ratio of 1.7 to 1.9. It can be directly used for enzyme digestion and sequencing.
Application
Suitable for fast λphage DNA
