miEASY microRNA Kit
2024-12-27Allprep RNA/DNA/miRNA Extract Kit
2024-12-27Product Number: RNK4701
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK4701 50Preps |
| Buffer RLT Plus | RT | 50 ml |
| Buffer WS1 | RT | 12 ml |
| Buffer WS 2/3 | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| Buffer IR | RT | 25 ml |
| Buffer WB | RT | 15ml |
| Buffer APP | RT | 60 ml |
| Buffer EB | RT | 10 ml |
| Genomic DNA adsorption column DA and collection tube | RT | 50 |
| RNA adsorption column RA and collection tube | RT | 50 |
Description
This kit is designed to rapidly extract and isolate genomic DNA, as well as total RNA and Proten, including miRNA, from the same animal cell or tissue sample. The unique Buffer RLT Plus rapidly cleaves and inactivates cellular RNA/DNA enzymes, and then cleaves the mixture of RNA/miRNA/DNA/Protein while passing through a genomic DNA adsorption column, where genomic DNA is adsorbed and miRNA/RNA/Protein penetrates and filters through. The genomic DNA on the DNA adsorption column undergoes a series of rinsing centrifugation to obtain pure genomic DNA. After adjusting the binding conditions with ethanol, the filtered miRNA/RNA selectively adsorbs onto the miRNA/RNA adsorption column in a highly dissociated salt state, and then obtains pure miRNA/RNA through a series of rapid rinsing centrifugal elution. The filtrate was selectively precipitated to obtain Protein. On the basis of phenol free and chloroform free DNA/RNA rapid extraction technology, combined with exclusive separation technology, the miRNA/RNA/genomic DNA obtained simultaneously has high purity and does not interfere with each other. The obtained miRNA/RNA does not require DNase digestion and can be directly used for experiments such as reverse transcription PCR and fluorescence quantitative PCR. Genomic DNA can also be directly used for various downstream experiments such as Southern, enzyme digestion, PCR, etc.
Features
1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2.Fast and simple, the separation of miRNA/RNA/genomic DNA/Protein from a single sample can generally be completed within 1 hour.
3.The exclusive adsorption column and formula ensure effective removal of genomic DNA residues. Generally, the miRNA/RNA obtained does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments.
4.Multiple column washes ensure high purity of miRNA/RNA/genomic DNA, which can be directly used in various downstream experiments.
Application
Suitable for rapid extraction and separation of genomic DNA and total RNA and Protein containing miRNA from the same animal cell or tissue sample (such as purchasing additional miRNA adsorption columns and matching solutions, and can also separate and extract total RNA and miRNA from the same sample to enrich miRNA), without the need for DNase digestion, RNA can be directly used for reverse transcription PCR and fluorescence quantitative PCR.
