Manual

Product Number: DNK1601

Shipping and Storage

1. When the ambient temperature is low, some detergent ingredients in Buffer YL will precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes and gently shaken to restore clarity. Do not shake violently to avoid excessive foam formation.
2. Buffer PP may experience precipitation and precipitation, and can be re dissolved by taking a water bath at 37℃ for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

When cells undergo apoptosis, chromatin DNA breaks between nucleosomes, ultimately forming DNA fragments with integer multiples of 200bp. These DNA fragments are extracted, and after electrophoresis and ethidium bromide staining, they form a ladder like appearance, known as DNA Ladder. After the lysis of blood and tissue cultured cells in Buffer CB, the released DNA fragments selectively adsorb onto the silica matrix membrane in a highly dissociated salt state. Then, through a series of rapid rinsing centrifugation steps, impurities such as salt, cell metabolites, and proteins are removed. The lowest salt buffer EB washes the DNA Ladder fragments off the silica matrix membrane.

Features

1. No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
2. Our company's unique lysis/binding solution formula effectively lyses cells, and using this reagent kit does not require expensive protease K treatment, greatly reducing usage costs and accelerating processing speed.
3. Time saving, concise, and single sample operation can generally be completed within 10 minutes.

Application

Suitable for rapid extraction of apoptotic DNA Ladder

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