Genomic Fast DNA Kit
2024-12-02Bacterial Genomic DNA Kit(liquid form)
2024-12-02Product Number: DNK1101
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37 ℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Proteinase K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25°C for at least 6 months, at 4°C for 12 months, and at -20°C for 2 years.
- To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK1101 50 Preps | DNK1102 100 Preps | DNK1103 200 Preps |
| Balance Buffer | RT | 5 ml | 10 ml | 20 ml |
| Buffer RB | RT | 30 ml | 60 ml | 120 ml |
| Buffer CB | RT | 11 ml | 20 ml | 40 ml |
| Buffer IR | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 20 ml |
| Proteinase K | 4°C | 1 ml | 1ml ×2 | 1ml ×4 |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The unique Buffer CB/Proteinase K rapidly cleaves cells and inactivates intracellular nucleases, and then selectively adsorbs genomic DNA onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer IR and Buffer WB remove impurities such as cellular metabolites and proteins. Finally, low salt Buffer EB elutes pure genomic DNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Fast and simple, single sample operations can generally be completed within 30 minutes.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.7-1.9 and a length of up to 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
