Bacterial Genomic DNA Fast Kit
2024-12-02Bacterial genomic Medium Kit
2024-12-02Product Number: DNK1201
Shipping and Storage
- When the ambient temperature is low, some detergent ingredients in Buffer NLF will precipitate and become turbid or precipitate. It can be heated in a 37°C water bath for a few minutes, and then gently shaken to restore clarity. Do not shake violently to avoid excessive foam formation.
- Buffer PP may experience precipitation and precipitation, and can be re dissolved in a water bath at 37°C for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK1201 50 Preps | DNK1202 100 Preps | DNK1203 200 Preps |
| Buffer NLF | RT | 30 ml | 60 ml | 120 ml |
| Buffer PP | RT | 10 ml | 20 ml | 40 ml |
| Buffer DD | RT | 10 ml | 15ml | 30 ml |
| RNaseA(10mg/ml) | -20°C | 150μl | 250μl | 400μl |
Description
This kit is used for rapid extraction of genomic DNA from various bacteria. After adding bacterial samples to Buffer NLF (or using lysozyme or other enzymes to help break down cell walls), the cells are first lysed under strong detergent action to release genomic DNA. RNase A is then added to remove RNA, followed by selective precipitation of buffer PP to remove proteins. Finally, pure genomic DNA is precipitated in isopropanol and re dissolved in Buffer DD.
Features
- No need to use toxic reagents such as phenol.
- Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
- The results are stable and the yield is high. The typical ratio of OD260/OD280 is 1.7~1.9, and the length can reach 50-150kb. It can be directly used for library construction, PCR, Southern blot, and various alcohol cleavage reactions.
Application
Used for rapid extraction of various bacterial genomic DNA
