Manual

Product Number: DNK0301

Shipping and Storage

1. When the ambient temperature is low, some detergent ingredients in Buffer NLY will precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
2. Buffer PP may experience precipitation and precipitation, and can be re dissolved by taking a water bath at 37℃ for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This kit uses several rapid steps to extract genomic DNA based on the characteristics of whole blood. Firstly, Buffer ELY cleaves red blood cells without DNA, while Buffer NLY cleaves white blood cells to release genomic DNA. Then, Buffer PP selectively precipitates to remove proteins. Finally, pure genomic DNA is precipitated in isopropanol and re dissolved in Buffer DA.

Features

1. The Buffer ELY formula selected from over a dozen formulas is fast and complete in cracking.
2. No need to use toxic reagents such as phenol.
3. Fast and simple, the operation of a single sample can generally be completed within 1 hour.
4. The results are stable and the yield is high (typical yield of 3ml whole blood can extract 50-150µg). The OD260/OD280 typical ratio is 1.7-1.9, and the length can reach 50Kb-150kb. It can be directly used for library construction, PCR, Southern blot, and various enzyme digestion reactions.

Application

Suitable for rapid extraction of genomic DNA from whole blood of various animals

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