50bp DNA Ladder marker (5μl/T)
2024-11-29
Blood and Tissue DNA Mini Kit,50 preps
2024-12-02Product Number: DNK0101
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Proteinase K is stored in a ready-to-use glycerol buffer and transported at room temperature. Upon receipt, it should be stored at room temperature for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years
- Avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air. Each solution should be covered tightly after use.
Components
| Component | Storage | DNK0101 50 Preps | DNK0102 100 Preps | DNK0103 200 Preps |
| Balance Buffer | RT | 5ml | 10ml | 20ml |
| Buffer CB | RT | 15ml | 30ml | 60ml |
| Buffer IR | RT | 25ml | 50ml | 100ml |
| Buffer WB | RT | 13ml | 25ml | 50ml |
| Buffer EB | RT | 15ml | 15ml | 20ml |
| Proteinase K | RT | 1ml | 1ml×2 | 1ml×4 |
| Adsorption column AC | 4°C | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The unique Buffer CB and Proteinase K rapidly lyse cells and inactivate intracellular nucleases, and then selectively adsorb genomic DNA onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer IR and Buffer WB remove impurities such as cell metabolites and proteins. Finally, low salt Buffer EB elutes pure genomic DNA from the silica matrix membrane.
Features
- It does not require the use of toxic reagents such as phenol, nor does it require steps such as ethanol precipitation.
- Fast and simple, the operation of a single sample can generally be completed within 20 minutes.
- Multiple column washes ensure high purity, with a typical yield of 200µl of whole blood, which can extract 3-12µg, and a typical OD260/OD280 ratio of 1.7-1.9, with a length of up to 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
- The red blood cell lysis fluid formula selected from over a dozen formulas is fast and complete, and customers can choose to purchase it according to their needs.
- A typical yield of 200µl of whole blood can extract 3-12µg of genomic DNA.
Application
Suitable for rapid extraction of whole blood genomic DNA
