Bacterial genomic Medium Kit
2024-12-02Product Number: DNK1401
Shipping and Storage
1.Buffer PL or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again by taking a water bath at 55℃ for a few minutes, restoring clarity and transparency, and then cooled to room temperature before use. The concentration of Guanidine Hydrochloride in Buffer PQ is high, and there may be some precipitation after adding ethanol, which does not affect its use. Take the supernatant directly and use it.
2.Avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air. Each solution should be covered tightly after use.
Components
| Component | Storage | DNK1401 50 Preps | DNK1401 100 Preps | DNK1401 200 Preps |
| Buffer PL | RT | 30 ml | 60 ml | 120 ml |
| Buffer PQ | RT | 18 ml | 35 ml | 70 ml |
| Buffer IR | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 20 ml |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The improved classic CTAB plant DNA extraction solution (with the addition of various polysaccharide and polyphenol removal components tailored to plant characteristics) rapidly lyses cells and inactivates intracellular nucleases. After chloroform extraction, polysaccharides, polyphenols, and proteins are removed by centrifugation (as needed, isopropanol is also added to the supernatant to precipitate genomic DNA and further remove other impurities), then, the genomic DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as polysaccharides, polyphenols, cellular metabolites, proteins, etc. are further removed. Finally, pure genomic DNA is eluted from the silica matrix membrane in a low salt elution buffer.
Features
1.The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2.No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
3.Fast and simple, the operation of a single sample can generally be completed within 1 hour.
4.Several types of polysaccharides and polyphenols are removed, and multiple column washes are used to ensure high purity. The typical OD260/OD280 ratio is 1.7-1.9, with a length of up to 20kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
Application
Suitable for rapid extraction of plant tissues (including complex polysaccharide and phenolic plants) and fungal genomic DNA.
