PLANT RNA Fast Kit
2024-12-27EASYall RNA/DNA/Protein Mini Kit
2024-12-27DNase Digestion Kit
Product Number: RNK3401
Shipping and Storage
Store DNase buffer at -20℃, buffer RW1 at room temperature or 4℃, RNase free DNase at -20℃ to avoid repeated freeze-thaw cycles.
Component
| Component | Storage | RNK3401 50preps |
| DNase Buffer | -20℃ | 1.25 ml×2 |
| RNase free DNaseI | -20℃ | 0.25 ml |
| Buffer RW1 | RT | 40 ml |
Description
Any total RNA extraction reagent cannot completely avoid trace residues of DNA during the extraction process. Our EASYspin series RNA extraction products, due to our unique buffer system and special silica gel adsorption membrane, can remove the vast majority of DNA contamination, so DNaseI digestion is generally not required. However, for some sensitive downstream experiments, it is necessary to remove trace amounts of DNA residue. You can purchase our DNase Digestion Kit and directly digest the remaining DNA on a centrifuge adsorption column. Then, pure RNA can be washed off and used directly. This product is compatible with all silica gel membrane centrifuge column RNA extraction kits.
Feature
- Simple and fast, with optimized conditions, it can generally digest and remove residual DNA on the silicone membrane in 15 minutes.
- Ensuring RNase free can ensure the integrity of RNA molecules.
- Wide compatibility, can be integrated into all silica gel membrane centrifuge column RNA extraction kit columns for digestion, without the need to extract total RNA and then remove residual DNA separately.
Note
DNase is very sensitive and prone to physical damage, denaturation, and loss of activity, so do not mix DNase I and working fluid evenly. Gently blow or invert to mix well. Prepare fresh working waves in advance during RNA extraction. DNaseI Buffer is specifically designed for column digestion in conjunction with RNase free DNaseI. A typical 10 x DNase Buffer cannot be used for membrane DNase digestion and cannot be replaced.
