DNase Digestion Kit
2024-12-27EASYspin Plant RNA Max Kit
2024-12-27Product Number: RNK3602
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK360250 Preps |
| Buffer RLT | RT | 50 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10ml |
| RNase-free H2O | RT | 10 ml |
| 70% ethanol | RT | 9ml RNase-free H2O |
| Buffer IR | RT | 25 ml |
| Buffer WB | RT | 13ml |
| Buffer APP | RT | 60 ml |
| Buffer EB | RT | 10 ml |
| Genomic DNA adsorption column DA and collection tube | RT | 50 |
| RNA adsorption column RA and collection tube | RT | 50 |
Description
This reagent kit is designed to rapidly extract and isolate genomic DNA, total RNA, and protein simultaneously from the same animal cell or tissue sample. The unique Buffer RLT rapidly cleaves cells and inactivates cellular RNA/DNA enzymes, then cleaves the mixture of DNA/RNA/Protein while passing through a genomic DNA adsorption column, where genomic DNA is adsorbed and RNA/Protein penetrates through filtration. The genomic DNA on the DNA adsorption column undergoes a series of rinsing centrifugation to obtain pure genomic DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the RNA adsorption column in a highly dissociated salt state, and then obtains pure RNA through a series of rapid rinsing centrifugation elution. Protein was obtained by selective precipitation of the filtrate. On the basis of phenol free and chloroform free DNA/RNA rapid extraction technology combined with exclusive separation technology, the RNA/genomic DNA obtained simultaneously has high purity and does not interfere with each other. The obtained RNA does not require DNase digestion and can be directly used for experiments such as reverse transcription PCR and fluorescence quantitative PCR. Genomic DNA can also be directly used for various downstream experiments such as Southern, enzyme digestion, PCR, etc.
Features
1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2.Fast, simple, and single sample RNA/genomic DNA/Protein separation operations can generally be completed within 1 hour.
3.The exclusive adsorption column and formula of the reagent kit ensure effective removal of genomic DNA residues. Generally, the obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments.
4.Multiple column washes ensure high purity of RNA/genomic DNA, which can be directly used in various downstream experiments.
Application
Suitable for rapid extraction and isolation of genomic DNA, total RNA, and protein from the same animal cell or tissue sample, without the need for DNase digestion. RNA can be directly used for reverse transcription PCR and fluorescence quantitative PCR.
