EASYall RNA/DNA/Protein Mini Kit

Product Number: RNK3602

Shipping and Storage 

1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.

2.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).

3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	RNK360250 Preps
Buffer RLT	RT	50 ml
Buffer RW1	RT	40 ml
Buffer RW	RT	10ml
RNase-free H2O	RT	10 ml
70% ethanol	RT	9ml RNase-free H2O
Buffer IR	RT	25 ml
Buffer WB	RT	13ml
Buffer APP	RT	60 ml
Buffer EB	RT	10 ml
Genomic DNA adsorption column DA and collection tube	RT	50
RNA adsorption column RA and collection tube	RT	50

Description

This reagent kit is designed to rapidly extract and isolate genomic DNA, total RNA, and protein simultaneously from the same animal cell or tissue sample. The unique Buffer RLT rapidly cleaves cells and inactivates cellular RNA/DNA enzymes, then cleaves the mixture of DNA/RNA/Protein while passing through a genomic DNA adsorption column, where genomic DNA is adsorbed and RNA/Protein penetrates through filtration. The genomic DNA on the DNA adsorption column undergoes a series of rinsing centrifugation to obtain pure genomic DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the RNA adsorption column in a highly dissociated salt state, and then obtains pure RNA through a series of rapid rinsing centrifugation elution. Protein was obtained by selective precipitation of the filtrate. On the basis of phenol free and chloroform free DNA/RNA rapid extraction technology combined with exclusive separation technology, the RNA/genomic DNA obtained simultaneously has high purity and does not interfere with each other. The obtained RNA does not require DNase digestion and can be directly used for experiments such as reverse transcription PCR and fluorescence quantitative PCR. Genomic DNA can also be directly used for various downstream experiments such as Southern, enzyme digestion, PCR, etc.

Features

1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.

2.Fast, simple, and single sample RNA/genomic DNA/Protein separation operations can generally be completed within 1 hour.

3.The exclusive adsorption column and formula of the reagent kit ensure effective removal of genomic DNA residues. Generally, the obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments.

4.Multiple column washes ensure high purity of RNA/genomic DNA, which can be directly used in various downstream experiments.

Application

Suitable for rapid extraction and isolation of genomic DNA, total RNA, and protein from the same animal cell or tissue sample, without the need for DNase digestion. RNA can be directly used for reverse transcription PCR and fluorescence quantitative PCR.

Note

1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000 rpm.

2.The sample processing capacity should never exceed the processing capacity of the genomic adsorption column DA and RNA adsorption column RA, otherwise it may cause DNA residue or a decrease in production. There is a significant difference in RNA/DNA among different types of tissue cells, for example, the thymus and spleen have abundant DNA content, exceeding 5mg will exceed the column processing capacity. COS cells have abundant RNA content, exceeding 3×10⁶ cells will exceed the column adsorption capacity. So when starting to explore the experimental conditions, if the DNA/RNA content of the sample is not clear, it is better to use a smaller sample processing volume, such as cells not exceeding 3-4×10⁶ and tissues not exceeding 10mg. In the future, the processing capacity will be increased or decreased based on the sample testing situation.

3.Buffer RLT, Buffer IR, and Buffer RW1 contain irritating compounds. When operating, latex gloves should be worn to avoid contact with skin, eyes, and clothing. If it gets on the skin or eyes, rinse with plenty of water or physiological saline.

4.If this reagent kit is needed for the extraction of DNA/RNA/Protein from plant samples, especially difficult samples with abundant secondary metabolites of polysaccharides and polyphenols, please consult technical personnel as other reagents may be required.

5.Regarding trace residues of DNA:

Generally speaking, any total RNA extraction reagent cannot completely avoid trace residues of DNA during the extraction process (DNase digestion cannot achieve 100% residue free). Our company's EASY all RNA/DNA/Protein Mini Kit, due to its unique Buffer system and genomic DNA separation and purification technology, has cleared the vast majority of DNA and does not require DNase digestion. It can be directly used for reverse transcription PCR and fluorescence quantitative PCR. In some special cases, such as residual DNA caused by excessive DNA content or strict mRNA expression analysis requiring fluorescence quantitative PCR, we suggest that when selecting templates and primers:

5.1.Select primers that cross introns to pass through the connecting regions in mRNA, so that DNA cannot serve as a template for amplification reactions.

5.2.Select primer pairs with different sizes of amplified products on genomic DNA and cDNA.

5.3.Treat RNA extract with DNase I of RNase free to improve efficacy. This reagent kit can also be used for RNA cleaning after DNase I treatment. Please contact us for specific operating instructions.

5.4.Before rinsing Buffer RW1, perform DNase I treatment directly on the adsorption column RA. Please contact us to request specific operating instructions.

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