EASYspin Fast Blood RNA Kit

Product Number: RNK0602

Shipping and Storage 

1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37 ℃ water bath for a few minutes to restore clarity.

2.Inappropriate storage at low temperatures (4 ℃ or -20 ℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15 ℃ -25 ℃).

3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	RNK0602 50 Preps
10×Buffer RLB	RT	25 ml
Buffer RLT	RT	30 ml
Buffer RW1	RT	40 ml
Buffer RW	RT	10 ml
70% ethanol	RT	9ml RNase-free H2O
RNase-free H2O	RT	10 ml
RNase free adsorption column RA and collection tube	RT	50

Description

Buffer RLB selectively cleaves red blood cells, followed by unique Buffer RLT/ β- Mercaptoethanol rapidly cleaves white blood cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O elutes pure RNA from the silica matrix membrane.

Features

1.No toxic reagents such as phenol/chloroform are required, and no steps such as ethanol precipitation are required.

2.The repeatedly optimized Buffer RLB formula achieves fast and complete cracking effect.

3.Fast and simple, the operation of a single sample can generally be completed within 40 minutes.

4.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9-2.1 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.

Note

1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000 rpm.

2.Self prepared ethanol is required, β- Mercaptoethanol.

3.Buffer RLT and Buffer RW1 contain irritating compounds, and latex gloves should be worn during operation to avoid contact with skin, eyes, and clothing. If it gets on the skin or eyes, rinse with plenty of water or physiological saline.

4.Regarding trace residues of DNA:

5.Generally speaking, any total RNA extraction reagent cannot completely avoid trace DNA residues during the extraction process. Our company's EASYspin series RNA extraction products, due to the use of our unique buffer system and the selection of adsorption membranes with special adsorption capabilities, do not have a significant impact on extremely small amounts of DNA residues in most RT-PCR amplification processes. If strict mRNA expression level analysis, such as fluorescence quantitative PCR, is required, We suggest that when selecting templates and primers:

6.Select primers that cross introns to pass through the connecting regions in mRNA, so that DNA cannot serve as a template for amplification reactions. Alternatively, primer pairs with different sizes of amplified products on genomic DNA and cDNA can be selected. Shorten the extension time to prevent the DNA source template from participating in the amplification reaction.

7.Treat the RNA extract with DNase I of RNase free. This reagent kit can also be used for RNA cleaning after DNase I treatment. Please contact us for specific operating instructions.

8.Before rinsing buffer RW1, perform DNase I treatment (RNK3401: DNase Digestion Kit) directly on the adsorption column RA. Refer to Appendix 1.

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