miEASY microRNA Mini Kit
2024-12-27EASYspin RNA Mini Kit
2024-12-27Product Number: RNK0602
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37 ℃ water bath for a few minutes to restore clarity.
2.Inappropriate storage at low temperatures (4 ℃ or -20 ℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15 ℃ -25 ℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK0602 50 Preps |
| 10×Buffer RLB | RT | 25 ml |
| Buffer RLT | RT | 30 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10 ml |
| 70% ethanol | RT | 9ml RNase-free H2O |
| RNase-free H2O | RT | 10 ml |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
Buffer RLB selectively cleaves red blood cells, followed by unique Buffer RLT/ β- Mercaptoethanol rapidly cleaves white blood cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O elutes pure RNA from the silica matrix membrane.
Features
1.No toxic reagents such as phenol/chloroform are required, and no steps such as ethanol precipitation are required.
2.The repeatedly optimized Buffer RLB formula achieves fast and complete cracking effect.
3.Fast and simple, the operation of a single sample can generally be completed within 40 minutes.
4.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9-2.1 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
