Manual

Product Number: RNK4401

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3. To avoid reducing activity and facilitate transportation, protease K is provided as a freeze-dried powder. After receiving it, it can be briefly centrifuged and dissolved in 0.25ml (20 times) and 0.5ml (50 times) of RNase free H₂O. Because repeated freeze-thaw cycles may reduce enzyme activity, after dissolution, it can be immediately packaged and frozen according to the amount used each time, and stored at -20℃.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Component

Description

Unique Buffer RLT/ β-Mercaptoethanol rapidly cleaves cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cellular metabolites and proteins. Finally, low salt RNase free H2O washes pure RNA off the silica matrix membrane.

Feature

1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. No toxic reagents such as phenol and chloroform are needed, and no steps such as ethanol precipitation are required.
3. Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
4. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9-2.0 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.

Applications

Suitable for rapid extraction of tissue RNA rich in heart, skeletal muscle, blood vessels, trachea, skin, and other fibers

Get a quote