Manual

Product Number: RNK3001

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3. To avoid reducing activity and facilitate transportation, Proteinase K is provided as a freeze-dried powder. Upon receipt, it can be briefly centrifuged and dissolved in 0.5ml of sterilized water (final concentration 40mg/ml). Because repeated freeze-thaw cycles may reduce enzyme activity, immediately after dissolution, according to the amount used each time, pack and store at -20℃.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Component

Description

This kit is designed for rapid extraction of total RNA from formalin fixed and paraffin embedded tissue samples. The unique Buffer PKD/Proteinase K rapidly cleaves cells to release RNA, and then the lysis mixture passes through a genomic DNA clearance column, where genomic DNA is cleared and RNA penetrates through filtration. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Then, through a series of rapid rinsing centrifugation steps, the protein solution and Buffer RW are used to remove impurities such as cell metabolites and proteins. Finally, the pure RNA is eluted from the silica matrix membrane with low salt RNase free H₂O. The obtained RNA can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.

Features

1. No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2. Fast and simple, single sample RNA extraction can generally be completed within 1 hour.
3. The exclusive genome clearance column and formula of the reagent kit ensure effective clearance of genomic DNA residues. Generally, the obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments.
4. Multiple column washes ensure high purity of RNA and can be directly used for various downstream experiments.

Applications

Suitable for rapid extraction of total RNA from formalin fixed and paraffin embedded tissue samples, RNA can be used for reverse transcription PCR and fluorescence quantitative PCR.

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