Manual

Product Number: RNK3101

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2. Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3. Buffer WS2/3 may precipitate crystals after using ethanol for a few days, which does not affect its use. Simply do not absorb the crystals and use the supernatant.
4. Protease K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.
5. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This kit is designed for rapid extraction of RNA, including microRNA, from formalin fixed and paraffin embedded tissue samples. The unique Buffer PKD/Protease K rapidly cleaves cells to release RNA, and then the lysis mixture passes through a genomic DNA clearance column, where genomic DNA is cleared and RNA penetrates through filtration. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Then, through a series of rapid rinsing centrifugation steps, the protein solution and Buffer RW are used to remove impurities such as cell metabolites and proteins. Finally, the pure RNA is eluted from the silica matrix membrane using low salt RNase free H2O. The obtained RNA can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.

Features

1. No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2. Fast and simple, single sample RNA extraction can generally be completed within 1 hour.
3. The exclusive genome clearance column and formula of the reagent kit ensure effective clearance of genomic DNA residues. Generally, the obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments.
4. Multiple column washes ensure high purity of RNA and can be directly used for various downstream experiments.

Application

Suitable for rapid extraction of RNA, including microRNA, from formalin fixed and paraffin embedded tissue samples. RNA can be directly used for reverse transcription PCR and fluorescence quantitative PCR.

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