EASYspin Plus Plant RNA Max Kit
2024-12-27EASYspin Fibrous Tissue Fast RNA Kit
2024-12-27EASYspin Plus Bacterial Fast RNA Kit
Product Number: RNK4302
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | RNK430250preps |
| TE (PH8.0) | RT | 6 ml |
| Lysozyme | 4℃ | 20 mg |
| Buffer RLT Plus | RT | 25 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10ml |
| 70% ethanol | RT | 9ml RNase-free H2O |
| RNase-free H2O | RT | 10 ml |
| Genomic DNA clearance column and collection tube | RT | 50 |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
On the basis of the EASYspin phenol free and chloroform RNA rapid extraction technology launched by our company, we have also independently developed the genome DNA clearance column technology to ensure effective removal of gDNA residues. The obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR and other experiments. The unique Buffer RLT Plus rapidly cleaves cells and inactivates cellular RNA enzymes, and then the mixture is cleaved through a genomic DNA clearance column, where genomic DNA is cleared and RNA penetrates through filtration. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O washes the pure RNA off the silica matrix membrane.
Feature
1.No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
2.Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
3.The exclusive development of genomic DNA clearance column technology ensures effective clearance of gDNA residues. The obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR and other experiments.
4.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.0-2.2 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
Applications
Suitable for rapid extraction of bacterial total RNA, using unique genomic DNA clearance column technology to ensure effective removal of gDNA residues, without the need for DNase digestion. RNA can be directly used for reverse transcription PCR and fluorescence quantification PCR.
