EASYspin Plus complex Plant RNA Kit
2024-12-27RNA HS Assay kit
2024-12-27EASYspin Plus bone tissue RNA
Product Number: RNK5401
Shipping and Storage
- Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK5401 50preps |
| Buffer CLB | RT | 50 ml |
| PLANTaid | RT | 5 ml |
| Buffer RLT Plus | RT | 25 ml |
| Buffer RW1 | RT | 40 ml |
| Buffer RW | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| Genomic DNA clearance column and collection tube | RT | 50 |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
Bone tissue is hard, bone cell density is low, and the peripheral matrix contains a large amount of mucins (proteoglycans) and RNA, which are difficult to separate and cannot be high-quality extracted using the traditional Trizol method. This reagent kit uses a unique phenol/chloroform free lysis solution and adds multiple components to remove bone tissue proteoglycans. Meanwhile, the unique genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for reverse transcription PCR, fluorescence quantitative PCR, and other experiments. The unique Buffer CLB and Buffer RLT Plus rapidly lyse cells and inactivate cell RNA enzymes, centrifuge precipitate to remove polysaccharides and secondary metabolites, then lyse the mixture and regulate RNA binding with ethanol to adsorb onto the genomic DNA clearance column. RNA is selectively washed and filtered, and residual DNA adsorbed on the genomic DNA clearance column cannot be washed off and discarded along with the column to remove DNA. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H2O washes the pure RNA off the silica matrix membrane.
Features
- No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
- Simplicity, single sample operation can generally be completed within 35 minutes, making it the simplest and fastest reagent kit in the world.
- The independently developed genomic DNA clearance column technology can effectively remove gDNA residues, and the obtained RNA generally does not require DNase digestion and can be used for experiments such as reverse transcription PCR and fluorescence quantitative PCR.
- Widely adaptable, it can extract various bone tissues, including mineralized bone tissue.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.2 and almost no DNA residue. It can be used for RT-PCR, Northem blot, second-generation sequencing, and various experiments.
Application
Suitable for rapid extraction of total RNA from bone tissue cells, unique genomic DNA clearance column technology can effectively remove visible gDNA residues on electrophoresis. RNA can be used for reverse transcription PCR, fluorescence quantitative PCR, etc.
