EZ spin plus Maxi Tissue/cell fast RNA extraction kit
2024-12-27EASYspin Plus Bacterial Fast RNA Kit
2024-12-27Product Number: RNK4201
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
- Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | RNK4201 10preps |
| Buffer RLT | RT | 100 ml |
| Buffer RLT Plus | RT | 50 ml |
| Buffer RW1 | RT | 120 ml |
| Buffer RW | RT | 25 ml×2 |
| RNase-free H2O | RT | 10 ml |
| PLANTaid | 4℃ | 10 ml×2 |
| Genomic DNA clearance column and collection tube | RT | 10 |
| RNase free adsorption column RA and collection tube | RT | 10 |
Description
On the basis of our company's exclusive introduction of EASYspin phenol free and chloroform based rapid RNA extraction technology, we have also independently developed the genome DNA clearance column technology, which can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion and can be directly used in PCR, fluorescence quantitative PCR and other experiments. Unique Buffer RLT/ β- Mercaptoethanol rapidly cleaves cells and inactivates cell RNA enzymes. Plant RNA co extractant PLANTaid helps to bind to polysaccharide polyphenols and remove them by centrifugation. Then, the mixture is cleaved using ethanol to regulate RNA binding and adsorption onto the genomic DNA clearance column. The genomic DNA is cleared and RNA is selectively eluted and filtered. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Then, through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW are used to remove impurities such as cell metabolites and proteins. Finally, the pure RNA is eluted from the silica matrix membrane with low salt RNase free H2O.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- No toxic reagents such as phenol and chloroform are needed, and no steps such as ethanol precipitation are required.
- Fast and simple, the operation of a single sample can generally be completed within 60 minutes.
- The unique plant RNA extractant can effectively bind polysaccharides and polyphenols, improving the clearance effect.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9-2.0 and almost no DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.
Applications
Suitable for rapid extraction of total RNA from plant tissue cells, the use of unique genomic DNA clearance column technology can effectively remove DNA residues, generally without the need for DNase digestion. RNA can be directly used for PCR and fluorescence quantitative PCR.
