Plasmid Mid Kit Liquid Form
2024-12-17Plasmid Maxi Kit
2024-12-17Product Number: PLK1001
Shipping and Storage
- when used for the first time, add all RNase A carried by the kit to buffer P1 (final concentration 100μg/ml) were stored at 2-8℃. If RNase A is inactivated in buffer P1, the extracted plasmid may have trace RNA residue. Add RNase A to buffer P1.
- when the ambient temperature is low, SDS in buffer P2 may precipitate turbidity or precipitation. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
- avoid volatilization, oxidation and pH change of reagents exposed to air for a long time, and close the cover of each solution in time after use.
- Buffer Er is transported at room temperature. It can be stored at 4℃ for one month, and stored at -20℃ for a long time.
Components
| Component | Storage | PLK1001 50 Preps |
| Balance Buffer | RT | 5ml |
| RNaseA(10mg/ml) | -20℃ | 250µl |
| Buffer P1 | 4℃ | 25 ml |
| Buffer P2 | RT | 25 ml |
| Buffer N3 | RT | 25 ml |
| Buffer PE | RT | 16 ml |
| Buffer ER | -20℃ | 10 ml |
| Buffer WB | RT | 15 ml |
| Buffer EB | RT | 15ml |
| Adsorption column AC | RT | 50 |
| Collection tube (2ml) | RT | 50 |
Description
This kit uses an improved SDS alkaline lysis method to lyse cells. Endotoxin is removed by a unique buffer Er selective combination with centrifugation. Then the silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components are removed by buffer PE and buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane by buffer EB with low salt and high pH.
Features
- The silicon matrix membranes in the centrifugal adsorption column are all specially made adsorption membranes imported from world-famous companies, with minimal difference in adsorption capacity between columns and good repeatability. It overcomes the disadvantage of unstable quality of domestic kit membrane.
- The unique process formula can remove endotoxin, and the endotoxin content is very low (<0.1eu/μg DNA), and the cell transfection effect was excellent. It can also be directly used in enzyme digestion, transformation, PCR, in vitro transcription, sequencing, and other molecular biology experiments.
