High Pure Plasmid Mini Kit
2024-12-16Yeast Plasmid Mini Kit
2024-12-17EndoFree Plasmid Mini Kit
Product Number: PLK0402
Shipping and Storage
- When using for the first time, add all RNase A carried by the reagent kit to Buffer P1 (final concentration 100ug/ml) and store at 2-8℃. If RNase A is inactivated in Buffer P1, there may be trace RNA residues in the extracted plasmid. Adding RNase A to Buffer P1 is sufficient.
- When the ambient temperature is low, SDS in Buffer P2 may precipitate turbidity or sediment. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
- Buffer ER can be transported at room temperature and stored at 4 ℃ for one month. It can be stored for a long time at -20 ℃.
Components
| Component | Storage | PLK0402 50 Preps |
| Balance Buffer | RT | 5ml |
| RNaseA(10mg/ml) | -20℃ | 150µl |
| Buffer P1 | 4℃ | 15 ml |
| Buffer P2 | RT | 15 ml |
| Buffer N3 | RT | 15 ml |
| Buffer PE | RT | 16 ml |
| Buffer ER | -20℃ | 5ml |
| Buffer WB | RT | 15 ml |
| Buffer EB | RT | 15ml |
| Adsorption column AC | RT | 50 |
| Collection tube (2ml) | RT | 50 |
Description
This reagent kit uses an improved SDS alkaline lysis method to lyse cells. The unique Buffer ER selectively binds to centrifugation to remove endotoxins. Then, the silica matrix membrane in the centrifugation adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components are removed through Buffer PE and Buffer WB. Finally, the pure plasmid DNA is washed off the silica matrix membrane with low salt and high pH Buffer EB.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- The unique process formula eliminates endotoxins, with extremely low endotoxin content (<0.1EU/μg DNA) and excellent cell transfection effect. It can also be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
