Inappropriate storage at low temperatures (4℃ or-20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃-25℃).
To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
This reagent kit should be stored at room temperature for 12 months without affecting its effectiveness. Poly Carrier can be transported at room temperature and stored at 4℃ for one month. It can be stored for a long time at -20℃
Components
Component
Storage
RNK3901 50Preps
Buffer RLT Plus
RT
25 ml
Buffer RW1
RT
40 ml
Buffer RW
RT
10 ml
70% ethanol
RT
9 ml
RNase-free H2O
RT
10 ml
Poly Carrier
-20℃
200 μl
Universal column and collection tube for micro DNA clearance/RNA adsorption
RT
100
Micro grinding pestle
RT
3
Description
This product is a specialized reagent kit for extracting trace amounts of RNA. Suitable for extracting total RNA from trace amounts of animal cells, micro cut tissues, and easily lysed animal tissues. The processing range is generally cells (<106) or tissues (<5mg). The exclusive DNA clearance/RNA adsorption universal column technology, combined with a special reagent formula that does not require DNA enzyme digestion, effectively removes gDNA residues. The obtained RNA has no DNA residue and can be directly used for downstream reverse transcription fluorescence quantitative PCR or high-throughput sequencing library construction experiments.
Features
No toxic reagents such as phenol and chloroform are used, and no steps such as ethanol precipitation are required.
Fast and simple, the operation of a single sample can generally be completed within 10 minutes.
The exclusive development of DNA clearance/RNA adsorption universal column technology ensures effective removal of gDNA residues. The obtained RNA does not require DNase digestion and can be directly used for reverse transcription fluorescence quantitative PCR, high-throughput sequencing library construction and other experiments.
Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of up to 2.0-2.2 and minimal DNA residue. It can be used for RT-PCR, Northern blot, and various experiments.