Virus RNA Fast Kit
2024-12-27EASYall RNA/DNA Mini Kit
2024-12-27Product Number: RNK2802
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity
2.Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK280250 Preps |
| Buffer RLT Plus | RT | 50ml |
| Buffer RW1 | RT | 40ml |
| Buffer RW | RT | 10ml |
| RNase-free H2O | RT | 10ml |
| 70% ethanol | RT | 9ml RNase-free H2O |
| Genomic DNA clearance column and collection tube | RT | 50 |
| RNase free adsorption column RA and collection tube | RT | 50 |
Description
On the basis of the exclusive launch of EZ spin phenol free and chloroform based rapid RNA extraction technology, our company has also independently developed a genome DNA clearance column technology to ensure effective removal of gDNA residues. The obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR and other experiments. The unique Buffer RLT Plus rapidly cleaves cells and inactivates cellular RNA enzymes, and then the mixture is cleaved through a genomic DNA clearance column, where genomic DNA is cleared and RNA penetrates through filtration. After adjusting the binding conditions with ethanol, the filtered RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Then, through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase-free H2O elutes pure RNA from the silica matrix membrane.
Features
1.Completely do not use toxic reagents such as phenol, chloroform, Beta mercaptoethanol, and do not require steps such as ethanol precipitation.
2.Fast and simple, the operation of a single cell sample can generally be completed within 15 minutes.
3.The exclusive development of genomic DNA clearance column technology ensures effective clearance of gDNA residues. The obtained RNA does not require DNase digestion and can be directly used for reverse transcription PCR, fluorescence quantitative PCR and other experiments.
4.Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.1-2.2 (the ratio of 100% pure RNA is generally around 2.2. Many companies' products have reduced ratios due to residual proteins or DNA, which cannot meet the purity standard of 2.2. Therefore, reducing the requirement by 1.9-2.0 is sufficient for use, but our product standards can generally reach high levels of 2.1-2.2 purity).
Application
Suitable for rapid extraction of total RNA from ordinary animal cells and easily lysed animal tissues, using unique genomic DNA clearance column technology to ensure effective clearance of gDNA residues, without the need for DNase digestion, RNA can be directly used for reverse transcription fluorescence quantitative PCR, Downstream experiments such as Northern blot.
