miEASY microRNA Blood/Serum/Plasma Kit
2024-12-27Glycogen DNA/RNA Carrier
2024-12-27Product Number: RNK2501, RNK2502
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
- Inappropriate storage at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃). Buffer RL can be transported at room temperature and stored in dark at 4 ℃ upon receipt.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | RNK2501 20Preps | RNK2502 50Preps |
| 10×Buffer RLB | RT | 10 ml | 20ml |
| Buffer RL | 4°C light avoidance | 25 ml | 50 ml |
| Buffer RE | RT | 15ml | 25 ml |
| Buffer RW | RT | 5ml | 10 ml |
| RNase-free H2O | RT | 10 ml | 10 ml |
| 70% ethanol | RT | 4ml RNase-free H2O | 9ml RNase-free H2O |
| RNase free adsorption column RA | RT | 20 | 50 |
| Collection tube (2ml) | RT | 20 | 50 |
Description
The improved guanidine isothiocyanate/phenol one-step method cleaves cells and inactivates RNA enzymes. Then, the total RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RE and Buffer RW remove cell metabolites, proteins, and other impurities. Finally, low salt RNase free H2O elutes pure RNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step method reagents, RNA can be directly eluted from the centrifugation column without the need for isopropanol precipitation and ethanol washing process, avoiding the problem of over drying and difficult dissolution.
- The unique Buffer RL formula can effectively eliminate genomic contamination.
- Multiple rinses to remove proteins result in higher purity of extracted RNA.
- Effectively removed the content of 5S in total RNA and improved purity.
Application
Suitable for rapid extraction of high-purity total RNA from whole blood
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2024-12-27