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Fast Blood RNA kit
Product Number: RNK2502
Shipping and Storage
- Transportation and storage are carried out at room temperature (15°C-25°C). Buffer TRI can be transported at room temperature and stored in the dark at 4°C for a long time after receipt. Even if stored at room temperature for 3 months, it will not affect the quality of use.
- Avoid prolonged exposure of reagents to air, which can cause volatilization, oxidation, and changes in pH value. After using each solution, the lid should be tightly closed in a timely manner.
Components
| Component | Storage | RNK2502 50Preps |
| 10×Buffer TRIB | RT | 25mL |
| Buffer TRI | 4°C light avoidance | 50mL |
| Buffer PE | RT | 16mL |
| Buffer RW | RT | 10mL |
| RNase-free H2O | RT | 5mL |
| RNase free adsorption column RA | RT | 50 |
| Collection tube (2mL) | RT | 50 |
Description
First, use red blood cell lysis buffer to lyse fresh anticoagulated whole blood red blood cells to obtain white blood cells. Improved guanidine isothiocyanate/phenol one-step method (TRIzol method) is used to lyse cells and inactivate RNAses. Total RNA is then selectively adsorbed onto the silica matrix membrane in a highly ionized salt state, followed by a series of rapid rinsing centrifugation steps to remove cell metabolites, proteins, and other impurities from the protein solution and rinse solution. Finally, low salt RNase free water is used to elute pure RNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.
- Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step reagent, RNA can be directly eluted from the centrifugation column without the need for isopropanol precipitation and ethanol washing process, avoiding the problem of excessive drying and difficult dissolution.
- Buffer TRI corresponds to Thermo Fisher/Invitrogen's TRIzol, which can effectively eliminate genomic contamination. The effect is consistent with the import.
- Repeated rinsing to remove protein results in higher purity of RNA extraction.
- Effectively removed the content of 5S in total RNA and improved purity.
Application
Suitable for rapid extraction of high-purity total RNA from whole blood
