Product Number: DNK4501
Shipping and Storage
All reagents, when store in indicated temperature, are stable for 9 months.
Components
Component | Storage | DNK4501 50 Preps |
Bead Tube | RT | 50 |
Buffer SP | RT | 50 ml |
Buffer MT | RT | 6 ml |
Buffer PPS | RT | 13 ml |
Buffer IRS | RT | 15 ml |
Buffer PQ | RT | 35 ml |
Buffer WB | RT | 13 ml |
Buffer EB | RT | 10 ml |
DNA Bind Columns | RT | 50 |
Description
The FastBeat Soil DNA Kit quickly and efficiently isolates PCR-ready genomic DNAdirectly from soil samples in less than 40 minutes. Designed for use with Beads-Beating device such as the FastPrep® Instruments from MP Biomedicals,soil organisms population are easily lysed within 40 seconds. Samples are placed into 2.0 ml tubes containing 3 kinds of beads, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi. The kit uses a novel and proprietary method to remove high humic acid content including difficult soil types such as compost, sediment, and manure.The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae and Actinomycetes (e.g. Streptomyces).