Genomic Medium Kit
2024-12-02Bacterial Genomic DNA Fast Kit
2024-12-02Product Number: DNK1001
Shipping and Storage
1.Buffer TL, Buffer CB, or Buffer IR may precipitate and precipitate at low temperatures. They can be re dissolved in a water bath at 37°C for a few minutes, restored to clarity and transparency, and then cooled to room temperature before use.
2.Proteinase K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25°C for at least 6 months, at 4°C for 12 months, and at -20°C for 2 years.
3.To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK1001 50 Preps | DNK1002 100 Preps | DNK1003 200 Preps |
| Balance Buffer | RT | 5 ml | 10 ml | 20 ml |
| Buffer TL | RT | 11 ml | 20 ml | 40 ml |
| Buffer CB | RT | 15 ml | 30 ml | 60 ml |
| Buffer IR | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 20 ml |
| Proteinase K | 4°C | 1 ml | 1ml ×2 | 1ml ×4 |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The unique Buffer CB/Proteinase K rapidly lyses cells and uses a silica gel membrane centrifuge column to specifically adsorb DNA, without the need for toxic reagents such as phenol and chloroform, or time-consuming alcohol precipitation, maximizing the removal of proteins and other inhibitory impurities. Suitable for efficiently extracting genomic DNA from various materials (whole blood, animal tissue cells, mouse tail, Escherichia coli, etc.). The extracted DNA can be directly used for experiments such as enzyme digestion, PCR, Southern Blot, and virus detection.
Features
1.There is no need to use toxic reagents such as phenol or time-consuming ethanol precipitation.
2.Fast and simple, the Protocol of a single sample can generally be completed within 30 minutes.
3.Multiple column washes ensure high purity, with a typical yield of 200 µ l of whole blood capable of extracting 3-6 µ g of genomic DNA. The typical ratio of OD260/OD280 is 1.7-1.9, with a length of 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
4.The red blood cell lysis fluid formula selected from over a dozen formulas is fast and complete, and customers can choose to purchase it according to their needs.
Application
Suitable for anticoagulating whole blood/tissue/cells/mouse tail/bacteria and other genomic DNA.
