Manual

Product Number: DNK0901

Shipping and Storage

1. When the ambient temperature is low, some detergent ingredients in Buffer NLY will precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
2. Buffer PP may experience precipitation and precipitation, and can be re dissolved by taking a water bath at 37℃ for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
3. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

This kit is used for rapid extraction of genomic DNA from plant cells/tissues. After grinding or homogenizing the sample, buffer NLY is added. Firstly, cells are lysed under strong detergents or in synergy with Proteinase K to release genomic DNA. Then, RNase A is added to remove RNA, followed by selective precipitation of buffer PP to remove protein. Finally, pure genomic DNA is precipitated in isopropanol and re dissolved in buffer DA.

Features

1. No need to use toxic reagents such as phenol and chloroform.
2. Fast and simple, the entire process of organizing sample Protocols can be completed within 1 hour.
3. The results are stable and the yield is high (more than twice that of centrifugal column type), with a typical OD260/OD280 ratio of 1.7~1.9 and a length of 50kb-150kb. It can be directly used for PCR, Southern blot, various enzyme digestion reactions, and library construction.

Application

Suitable for rapid extraction of genomic DNA from various animal and plant cells/tissues

Get a quote