Manual

Product Number: PLK1201

Shipping and Storage

1. RNase A is stored in ready to use glycerol buffer solution, transported at room temperature, and upon receipt, stored at room temperature not exceeding 25℃ for at least 6 months, 4℃ for 12 months, and long-term storage at -20℃.
2. When used for the first time, all RNase A in the kit can be added to solution P1 (final concentration of 100μg/mL) and stored at 4℃ for about 3 months. If RNase A in solution P1 becomes inactive for a long time, there may be trace amounts of RNA residue in the extracted plasmid. Adding RNase A to solution P1 is sufficient.
3. SDS in solution P2 may precipitate when the ambient temperature is low, resulting in turbidity or precipitation. It can be heated in a 37℃ water bath for a few minutes to recover the clarification, and then remixed. Do not shake violently to avoid the formation of excessive foam.

Component

Component	Storage	10 preps
RNase A (10 mg/mL)	4℃	750μL
Buffer P1	4℃	77mL
Buffer P2	RT	77mL
Buffer N3	RT	77mL
Buffer PE	RT	64mL Add absolute ethanol before first use
Buffer WB	RT	50mL Add absolute ethanol before first use
Buffer EB	RT	20mL
Adsorption column DC	RT	10
Collection tube (50mL)	RT	10

This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This kit uses a unique high-yield SDS alkaline lysis formula to lyse cells, increasing plasmid yield by 1-2 times. The silicon matrix membrane inside the centrifugal adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Impurities and other bacterial components such as endotoxins are removed by passing protein solution and rinsing solution. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane using low salt and high pH elution buffer. The extracted plasmid has high purity and has removed most endotoxins. In addition to being used for routine PCR, enzyme digestion, transformation and other experiments, it can also be directly used for general transfection experiments such as protoplast transfection. For cell lines with special high requirements for transfection, Adlai's endotoxin free plasmid medium/large extraction kit can be selected.

Feature

1. The specially improved high-yield buffer formula can increase plasmid yield by 1-2 times.
2. All the silicon matrix membranes in the centrifugal adsorption column are made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawbacks of unstable membrane quality in domestic reagent kits.
3. The unique protein removal formula can efficiently remove residual nucleases, even strains with high nuclease content such as JM series and HB101 can be easily removed. Effectively prevent plasmids from being degraded by nucleases.
4. No toxic phenol, chloroform, or ethanol precipitation is required. Fast and convenient, 0.5-2 mg of pure high copy plasmid DNA can be quickly extracted from 150-300 ml of Escherichia coli LB (Luria Bertani) culture medium, with an extraction rate of 80-90%.

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