PhaseIso BAC DNA Kit
2024-12-17High Pure Yeast Plasmid Fast Maxi Kit
2024-12-17High Pure Plasmid Mid Scale Mini Kit
Product Number: PLK1801
Shipping and Storage
- When using for the first time, add all RNaseA carried by the reagent kit to Buffer P1 (final concentration 100μg/ml) and store at 2-8℃. If RNaseA is inactivated in Buffer P1, there may be trace RNA residues in the extracted plasmid. Adding RNaseA to Buffer P1 is sufficient.
- When the ambient temperature is low, SDS in Buffer P2 may precipitate turbidity or sediment. It can be heated in a 37 ℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | PLK1801 50 Preps |
| Balance Buffer | RT | 5ml |
| RNaseA（10mg/ml） | -20℃ | 250µl |
| Buffer P1 | 4℃ | 25ml |
| Buffer P2 | RT | 25ml |
| Buffer N3 | RT | 25ml |
| Buffer PE | RT | 16ml |
| Buffer WB | RT | 15ml |
| Buffer EB | RT | 15ml |
| Adsorption column AC | RT | 50 |
| Collection tube (2ml) | RT | 50 |
Description
This kit uses a unique high-yield SDS alkaline lysis formula to lyse cells, resulting in a 1-2 fold increase in plasmid production. The silicon matrix membrane in the centrifugal adsorption column selectively binds plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through Buffer PE and Buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane using low salt and high pH Buffer EB.
Features
- The specially improved high-yield buffer formula can increase plasmid production by 1-2 times.
- The unique Buffer PE formula can efficiently remove residual nucleases, even strains with abundant nuclease content such as JM series and HB101 can be easily removed. Effectively preventing plasmid degradation by nucleases.
- Fast and convenient, without the need for toxic reagents such as phenol and chloroform, and without the need for ethanol precipitation. The obtained plasmids have high yield and good purity, and can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
